Your browser doesn't support javascript.
loading
Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation.
Maron, Maxim I; Casill, Alyssa D; Gupta, Varun; Roth, Jacob S; Sidoli, Simone; Query, Charles C; Gamble, Matthew J; Shechter, David.
  • Maron MI; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, United States.
  • Casill AD; Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, United States.
  • Gupta V; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, United States.
  • Roth JS; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, United States.
  • Sidoli S; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, United States.
  • Query CC; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, United States.
  • Gamble MJ; Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, United States.
  • Shechter D; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, United States.
Elife ; 112022 01 05.
Article en En | MEDLINE | ID: mdl-34984976
ABSTRACT
Protein arginine methyltransferases (PRMTs) are required for the regulation of RNA processing factors. Type I PRMT enzymes catalyze mono- and asymmetric dimethylation; Type II enzymes catalyze mono- and symmetric dimethylation. To understand the specific mechanisms of PRMT activity in splicing regulation, we inhibited Type I and II PRMTs and probed their transcriptomic consequences. Using the newly developed Splicing Kinetics and Transcript Elongation Rates by Sequencing (SKaTER-seq) method, analysis of co-transcriptional splicing demonstrated that PRMT inhibition resulted in altered splicing rates. Surprisingly, co-transcriptional splicing kinetics did not correlate with final changes in splicing of polyadenylated RNA. This was particularly true for retained introns (RI). By using actinomycin D to inhibit ongoing transcription, we determined that PRMTs post-transcriptionally regulate RI. Subsequent proteomic analysis of both PRMT-inhibited chromatin and chromatin-associated polyadenylated RNA identified altered binding of many proteins, including the Type I substrate, CHTOP, and the Type II substrate, SmB. Targeted mutagenesis of all methylarginine sites in SmD3, SmB, and SmD1 recapitulated splicing changes seen with Type II PRMT inhibition, without disrupting snRNP assembly. Similarly, mutagenesis of all methylarginine sites in CHTOP recapitulated the splicing changes seen with Type I PRMT inhibition. Examination of subcellular fractions further revealed that RI were enriched in the nucleoplasm and chromatin. Taken together, these data demonstrate that, through Sm and CHTOP arginine methylation, PRMTs regulate the post-transcriptional processing of nuclear, detained introns.
Asunto(s)
Palabras clave

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteína-Arginina N-Metiltransferasas / Factores de Transcripción / Proteínas Nucleares / Intrones / Regulación de la Expresión Génica / Proteínas Nucleares snRNP Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Año: 2022 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteína-Arginina N-Metiltransferasas / Factores de Transcripción / Proteínas Nucleares / Intrones / Regulación de la Expresión Génica / Proteínas Nucleares snRNP Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Año: 2022 Tipo del documento: Article