Molecular detection of Mansonella mariae incriminates Simulium oyapockense as a potentially important bridge vector for Amazon-region zoonoses.
Infect Genet Evol
; 98: 105200, 2022 03.
Article
en En
| MEDLINE
| ID: mdl-34990852
ABSTRACT
OBJECTIVE:
To assess the emergent zoonotic disease risk posed by the voracious human-biting blackfly species Simulium oyapockense in the peripheral regions of an expanding urban centre situated deep in the Brazilian Amazon rainforest.METHODS:
We performed nine human landing catches at three periurban sites surrounding the Brazilian Amazon town of São Gabriel da Cachoeira. Using the detection of non-human primate filarial parasites as an indicator of the zoonotic disease threat posed by a biting insect, we screened 3328 S. oyapockense blackflies for the presence of zoonotic filarial DNA with an ITS-1 PCR assay and Sanger sequencing.RESULTS:
Between 98 and 100% of the biting insects captured during our nine collections were identified as S. oyapockense; at our three collection sites and during our three seasonally-distinct collections this species was captured at rates between 28 and 294 blackflies per hour. PCR screening of the march-collected S. oyapockense detected infectious-stage (L3) Mansonella mariae parasites (which are only known to infect non-human primates) in >0.15% of the tested head samples.CONCLUSIONS:
Our results show that residents of the periurban regions of São Gabriel da Cachoeira are routinely exposed to the bites of S. oyapockense blackflies which have previously fed on non-human primates.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Simuliidae
/
Zoonosis
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Insectos Vectores
/
Mansonella
/
Mansoneliasis
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Año:
2022
Tipo del documento:
Article