Effect of Denervation on XBP1 in Skeletal Muscle and the Neuromuscular Junction.
Int J Mol Sci
; 23(1)2021 Dec 24.
Article
en En
| MEDLINE
| ID: mdl-35008595
ABSTRACT
Denervation of skeletal muscle is a debilitating consequence of injury of the peripheral nervous system, causing skeletal muscle to experience robust atrophy. However, the molecular mechanisms controlling the wasting of skeletal muscle due to denervation are not well understood. Here, we demonstrate that transection of the sciatic nerve in Sprague-Dawley rats induced robust skeletal muscle atrophy, with little effect on the neuromuscular junction (NMJ). Moreover, the following study indicates that all three arms of the unfolded protein response (UPR) are activated in denervated skeletal muscle. Specifically, ATF4 and ATF6 are elevated in the cytoplasm of skeletal muscle, while XBP1 is elevated in the nuclei of skeletal muscle. Moreover, XBP1 is expressed in the nuclei surrounding the NMJ. Altogether, these results endorse a potential role of the UPR and, specifically, XBP1 in the maintenance of both skeletal muscle and the NMJ following sciatic nerve transection. Further investigations into a potential therapeutic role concerning these mechanisms are needed.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Nervio Ciático
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Músculo Esquelético
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Respuesta de Proteína Desplegada
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Proteína 1 de Unión a la X-Box
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Desnervación Muscular
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Unión Neuromuscular
Límite:
Animals
Idioma:
En
Año:
2021
Tipo del documento:
Article