Sensitive and real-time detection of IgG using interferometric reflecting imaging sensor system.
Biosens Bioelectron
; 201: 113961, 2022 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-35026547
ABSTRACT
Considering the limitations of well-known traditional detection techniques, innovative research studies have focused on the development of new sensors to offer label-free, highly sensitive, real-time, low-cost, and rapid detection for biomolecular interactions. In this study, we demonstrate immunoglobulin G (IgG) detection in aqueous solutions by using real-time and label-free kinetic measurements of the Interferometric Reflectance Imaging Sensor (IRIS) system. By performing kinetic characterization experiments, the sensor's performance is comprehensively evaluated and a high correlation coefficient value (>0.94) is obtained in the IgG concentration range of 1-50 µg/mL with a low detection limit (0.25 µg/mL or 1.67 nM). Moreover, the highly sensitive imaging system ensures accurate quantification and reliable validation of recorded binding events, offering new perspectives in terms of direct biomarker detection for clinical applications.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Inmunoglobulina G
/
Técnicas Biosensibles
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
2022
Tipo del documento:
Article