Mesoporous silica nanoparticles incorporated with zinc oxide as a novel antifungal agent against toxigenic fungi strains.

ABSTRACT

Developing environmentally friendly alternative strategies to reduce the damage caused by fungi in agriculture has been widely investigated. In this study, we evaluated using mesoporous silica nanoparticles (MSNs) incorporated with zinc oxide (MSNs-ZnO) as a potential antifungal agent against Fusarium graminearum and Aspergillus flavus strains, as well as their antimycotoxin properties. The MSNs that synthesized and characterized could release abundant ZnO in the first 24 h. Subsequently, the ZnO release became slower, providing greater durability of the antifungal effect. Significant (P < 0.001) growth reductions in F. graminearum (81%) and A. flavus (65%) compared to the control were obtained at a high concentration of the MSNs-ZnO (1.0 mg mL-1). Moreover, the MSNs-ZnO treatment at a high concentration (1.0 mg mL-1) caused morphology alteration in both fungi, showing ruptures and deformations in the fungal hyphae, affecting their growth and toxin production. A significant reduction (P < 0.001) in the productions of deoxynivalenol (89%) and aflatoxin B1 (58%) by F. graminearum and A. flavus were also observed. These findings imply that using MSNs as the carriers of zinc compounds, such as ZnO, could be investigated as a safe alternative for effectively controlling toxigenic fungi in agriculture.