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Genome-wide recombination map construction from single sperm sequencing in cattle.
Yang, Liu; Gao, Yahui; Li, Mingxun; Park, Ki-Eun; Liu, Shuli; Kang, Xiaolong; Liu, Mei; Oswalt, Adam; Fang, Lingzhao; Telugu, Bhanu P; Sattler, Charles G; Li, Cong-Jun; Cole, John B; Seroussi, Eyal; Xu, Lingyang; Yang, Lv; Zhou, Yang; Li, Li; Zhang, Hongping; Rosen, Benjamin D; Van Tassell, Curtis P; Ma, Li; Liu, George E.
  • Yang L; Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD, 20705, USA.
  • Gao Y; College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
  • Li M; Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD, 20705, USA.
  • Park KE; Department of Animal and Avian Sciences, University of Maryland, College Park, MD, 20742, USA.
  • Liu S; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
  • Kang X; Department of Animal and Avian Sciences, University of Maryland, College Park, MD, 20742, USA.
  • Liu M; Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD, 20705, USA.
  • Oswalt A; College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
  • Fang L; College of Agriculture, Ningxia University, Yinchuan, 750021, China.
  • Telugu BP; Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China.
  • Sattler CG; Select Sires Inc, 11740 U.S. 42 North, Plain City, OH, 43064, USA.
  • Li CJ; The Roslin Institute, Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Midlothian, EH25 9RG, UK.
  • Cole JB; Department of Animal and Avian Sciences, University of Maryland, College Park, MD, 20742, USA.
  • Seroussi E; Division of Animal Sciences, University of Missouri, Columbia, MO, 65201, USA.
  • Xu L; Select Sires Inc, 11740 U.S. 42 North, Plain City, OH, 43064, USA.
  • Yang L; Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD, 20705, USA.
  • Zhou Y; Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD, 20705, USA.
  • Li L; Agricultural Research Organization (ARO), Volcani Center, Institute of Animal Science, P.O.B 15159, HaMaccabim Road, 7528809, Rishon LeTsiyon, Israel.
  • Zhang H; Innovation Team of Cattle Genetic Breeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
  • Rosen BD; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Van Tassell CP; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Ma L; College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
  • Liu GE; College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
BMC Genomics ; 23(1): 181, 2022 Mar 05.
Article en En | MEDLINE | ID: mdl-35247961
ABSTRACT

BACKGROUND:

Meiotic recombination is one of the important phenomena contributing to gamete genome diversity. However, except for human and a few model organisms, it is not well studied in livestock, including cattle.

RESULTS:

To investigate their distributions in the cattle sperm genome, we sequenced 143 single sperms from two Holstein bulls. We mapped meiotic recombination events at high resolution based on phased heterozygous single nucleotide polymorphism (SNP). In the absence of evolutionary selection pressure in fertilization and survival, recombination events in sperm are enriched near distal chromosomal ends, revealing that such a pattern is intrinsic to the molecular mechanism of meiosis. Furthermore, we further validated these findings in single sperms with results derived from sequencing its family trio of diploid genomes and our previous studies of recombination in cattle.

CONCLUSIONS:

To our knowledge, this is the first large-scale single sperm whole-genome sequencing effort in livestock, which provided useful information for future studies of recombination, genome instability, and male infertility.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Recombinación Genética / Meiosis Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Recombinación Genética / Meiosis Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article