Improvement of the antibody-dependent respiratory burst assay for assessing protective immune responses to malaria.
Open Biol
; 12(3): 210288, 2022 03.
Article
en En
| MEDLINE
| ID: mdl-35291880
ABSTRACT
The antibody-dependent respiratory burst (ADRB) assay is a sensitive isoluminol-based chemiluminescence (CL) functional assay designed to assess the capacity of opsonizing antibodies against merozoites to induce neutrophil respiratory burst. ADRB was shown to measure protective immunity against malaria in endemic areas, but the assay needed further improvement to ensure better sensitivity and reproducibility. Here, we adjusted parameters such as the freezing-thawing procedure of merozoites, merozoites's concentration and the buffer solution's pH, and we used the improved assay to measure ADRB activity of 207 sera from 97 and 110 individuals living, respectively, in Dielmo and Ndiop villages with differing malaria endemicity. The improvement led to increased CL intensity and assay sensitivity, and a higher reproducibility. In both areas, ADRB activity correlated with malaria endemicity and individual's age discriminated groups with and without clinical malaria episodes, and significantly correlated with in vivo clinical protection from Plasmodium falciparum malaria. Our results demonstrate that the improved ADRB assay can be valuably used to assess acquired immunity during monitoring by control programmes and/or clinical trials.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Estallido Respiratorio
/
Malaria
Límite:
Animals
/
Humans
Idioma:
En
Año:
2022
Tipo del documento:
Article