Identification and Quantification by NMR Spectroscopy of the 22R and 22S Epimers in Budesonide Pharmaceutical Forms.
Molecules
; 27(7)2022 Mar 31.
Article
en En
| MEDLINE
| ID: mdl-35408662
ABSTRACT
The authors developed four variants of the qNMR technique (1H or 13C nucleus, DMSO-d6 or CDCl3 solvent) for identification and quantification by NMR of 22R and 22S epimers in budesonide active pharmaceutical ingredient and budesonide drugs (sprays, capsules, tablets). The choice of the qNMR technique version depends on the drug excipients. The correlation of 1H and 13C spectra signals to molecules of different budesonide epimers was carried out on the basis of a comprehensive analysis of experimental spectral NMR data (1H-1H gCOSY, 1H-13C gHSQC, 1H-13C gHMBC, 1H-1H ROESY). This technique makes it possible to identify budesonide epimers and determine their weight ratio directly, without constructing a calibration curve and using any standards. The results of measuring the 22S epimer content by qNMR are comparable with the results of measurements using the reference HPLC method.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Budesonida
/
Glucocorticoides
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
2022
Tipo del documento:
Article