Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin.
Nat Neurosci
; 25(5): 659-674, 2022 05.
Article
en En
| MEDLINE
| ID: mdl-35524141
ABSTRACT
The mouse visual system serves as an accessible model to understand mammalian circuit wiring. Despite rich knowledge in retinal circuits, the long-range connectivity map from distinct retinal ganglion cell (RGC) types to diverse brain neuron types remains unknown. In this study, we developed an integrated approach, called Trans-Seq, to map RGCs to superior collicular (SC) circuits. Trans-Seq combines a fluorescent anterograde trans-synaptic tracer, consisting of codon-optimized wheat germ agglutinin fused to mCherry, with single-cell RNA sequencing. We used Trans-Seq to classify SC neuron types innervated by genetically defined RGC types and predicted a neuronal pair from αRGCs to Nephronectin-positive wide-field neurons (NPWFs). We validated this connection using genetic labeling, electrophysiology and retrograde tracing. We then used transcriptomic data from Trans-Seq to identify Nephronectin as a determinant for selective synaptic choice from αRGC to NPWFs via binding to Integrin α8ß1. The Trans-Seq approach can be broadly applied for post-synaptic circuit discovery from genetically defined pre-synaptic neurons.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Ganglionares de la Retina
/
Colículos Superiores
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
2022
Tipo del documento:
Article