Assessing gene function in human B cells: CRISPR/Cas9-based gene editing and mRNA-based gene expression in healthy and tumor cells.
Eur J Immunol
; 52(8): 1362-1365, 2022 08.
Article
en En
| MEDLINE
| ID: mdl-35527391
ABSTRACT
Robust methods for manipulation of human B cells, isolated from healthy donors and patients with B cell disorders, has the potential to significantly accelerate B cell research. Our work describes a step-by-step protocol to perform electroporation-based screening of gene function in B cells through the use of Cas9 ribonuclecomplexes and in vitro produced mRNA.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Sistemas CRISPR-Cas
/
Edición Génica
Límite:
Humans
Idioma:
En
Año:
2022
Tipo del documento:
Article