Automated 96-well format high throughput colony formation assay for siRNA library screen.
STAR Protoc
; 3(2): 101355, 2022 06 17.
Article
en En
| MEDLINE
| ID: mdl-35542177
ABSTRACT
The colony formation assay is the gold-standard technique to assess cell viability after treatment with cytotoxic reagents, ionizing radiation, and cytotoxic combinatorial treatments. This protocol describes a high-throughput automated and high-content imaging approach to screen siRNA molecular libraries in HeLa cervical cancer cells in 96-well format. We detail reverse transfection of cells with siRNAs, followed by ionizing radiation, fixing, and staining of the plates for automated colony counting. This protocol can be used across a broad range of cell types. For complete details on the use and execution of this protocol, please refer to Tiwana et al. (2015).
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Radiación Ionizante
/
Ensayos Analíticos de Alto Rendimiento
Tipo de estudio:
Guideline
Idioma:
En
Año:
2022
Tipo del documento:
Article