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Whole-genome sequencing uncovers the structural and transcriptomic landscape of hexaploid wheat/Ambylopyrum muticum introgression lines.
Coombes, Benedict; Fellers, John P; Grewal, Surbhi; Rusholme-Pilcher, Rachel; Hubbart-Edwards, Stella; Yang, Cai-Yun; Joynson, Ryan; King, Ian P; King, Julie; Hall, Anthony.
  • Coombes B; Earlham Institute, Norwich, Norfolk, NR4 7UZ, UK.
  • Fellers JP; USDA-ARS Hard Winter Wheat Genetics Research Unit, Manhattan, Kansas, 66506, USA.
  • Grewal S; School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.
  • Rusholme-Pilcher R; Earlham Institute, Norwich, Norfolk, NR4 7UZ, UK.
  • Hubbart-Edwards S; School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.
  • Yang CY; School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.
  • Joynson R; Earlham Institute, Norwich, Norfolk, NR4 7UZ, UK.
  • King IP; School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.
  • King J; School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK.
  • Hall A; Earlham Institute, Norwich, Norfolk, NR4 7UZ, UK.
Plant Biotechnol J ; 21(3): 482-496, 2023 03.
Article en En | MEDLINE | ID: mdl-35598169
Wheat is a globally vital crop, but its limited genetic variation creates a challenge for breeders aiming to maintain or accelerate agricultural improvements over time. Introducing novel genes and alleles from wheat's wild relatives into the wheat breeding pool via introgression lines is an important component of overcoming this low variation but is constrained by poor genomic resolution and limited understanding of the genomic impact of introgression breeding programmes. By sequencing 17 hexaploid wheat/Ambylopyrum muticum introgression lines and the parent lines, we have precisely pinpointed the borders of introgressed segments, most of which occur within genes. We report a genome assembly and annotation of Am. muticum that has facilitated the identification of Am. muticum resistance genes commonly introgressed in lines resistant to stripe rust. Our analysis has identified an abundance of structural disruption and homoeologous pairing across the introgression lines, likely caused by the suppressed Ph1 locus. mRNAseq analysis of six of these introgression lines revealed that novel introgressed genes are rarely expressed and those that directly replace a wheat orthologue have a tendency towards downregulation, with no discernible compensation in the expression of homoeologous copies. This study explores the genomic impact of introgression breeding and provides a schematic that can be followed to characterize introgression lines and identify segments and candidate genes underlying the phenotype. This will facilitate more effective utilization of introgression pre-breeding material in wheat breeding programmes.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Triticum / Transcriptoma / Poaceae Idioma: En Año: 2023 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Triticum / Transcriptoma / Poaceae Idioma: En Año: 2023 Tipo del documento: Article