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Algoriphagus algorifonticola sp. nov., a marine bacterium isolated from cold spring area of South China Sea.
Yan, Cen; Chen, Can; Chai, Baozhong; Ye, Yanghui; Anwar, Nusratgul; Zhao, Zhe; Wang, Ruijun; Huo, Yingyi; Zhang, Xinyin; Wu, Min; Zheng, Daoqiong.
  • Yan C; Ocean College, Zhejiang University, Zhoushan 316000, PR China.
  • Chen C; Ocean College, Zhejiang University, Zhoushan 316000, PR China.
  • Chai B; College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
  • Ye Y; Ocean College, Zhejiang University, Zhoushan 316000, PR China.
  • Anwar N; College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
  • Zhao Z; College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
  • Wang R; Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Science, Huzhou University, Huzhou 313000, PR China.
  • Huo Y; College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
  • Zhang X; Ocean College, Zhejiang University, Zhoushan 316000, PR China.
  • Wu M; Ocean College, Zhejiang University, Zhoushan 316000, PR China.
  • Zheng D; College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Article en En | MEDLINE | ID: mdl-35604823
ABSTRACT
A Gram-stain-negative, aerobic, non-motile, short-rod-shaped bacterium, designated strain hg1T, was isolated from marine sediment within the cold spring area of South China Sea and subjected to a polyphasic taxonomic investigation. Colonies were circular and 1.0-2.0 mm in diameter, coral in colour, convex and smooth after growth on marine agar at 28 °C for 3 days. Strain hg1T was found to grow at 4-40 °C (optimum, 35-37 °C), at pH 6.5-9.0 (optimum, pH 7.5) and with 0-8 % (w/v) NaCl (optimum, 1.5-2 %). Chemotaxonomic analysis showed the sole respiratory quinone was MK-7, and the principal fatty acids are iso-C15  0, summed feature 3 (C16  1 ω7c and/or C16  1 ω6c), and iso-C16  0. The major polar lipids are phosphatidylethanolamine, an unidentified phospholipid and five unidentified glycolipids. The DNA G+C content of strain hg1T was 39.6 mol% based on the genome sequence. The comparison of 16S rRNA gene sequence similarities showed that hg1T was closely related to Algoriphagus ornithinivorans DSM 15282T (98.6 % sequence similarity), Algoriphagus zhangzhouensis MCCC 1F01099T (97.9 %) and Algoriphagus vanfongensis DSM 17529T (97.2 %); it exhibited 97.0 % or less sequence similarity to the type strains of other species of the genus Algoriphagus with validly published names. Phylogenetic trees reconstructed with the neighbour-joining, maximum-parsimony and maximum-likelihood methods based on 16S rRNA gene sequences showed that strain hg1T constituted a separate branch with A. ornithinivorans, A. zhangzhouensis, A. vanfongensis in a clade of the genus Algoriphagus. OrthoANI values between strain hg1T and A. ornithinivorans, A. zhangzhouensis and A. vanfongensis were 94.3, 74.1, 73.2 %, respectively, and in silico DNA-DNA hybridization values were 56.2, 18.5 and 18.3 %, respectively. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrated that strain hg1T is clearly distinct from recognized species of genus Algoriphagus. On the basis of these features, we propose that strain hg1T (=MCCC 1K03570T=KCTC 72111T) represents a novel species of the genus Algoriphagus with the name Algoriphagus algorifonticola sp. nov.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Agua de Mar / Ácidos Grasos País como asunto: Asia Idioma: En Año: 2022 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Agua de Mar / Ácidos Grasos País como asunto: Asia Idioma: En Año: 2022 Tipo del documento: Article