Structure and surface dynamics of genomic DNA as probed with surface-enhanced Raman spectroscopy: Trace level sensing of nucleic acids extracted from plants.
Spectrochim Acta A Mol Biomol Spectrosc
; 279: 121477, 2022 Oct 15.
Article
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| MEDLINE
| ID: mdl-35691169
ABSTRACT
In this work surface-enhanced Raman spectra of nucleic acids from in vitro grown Solanum tuberosum L. cultivars and populations (Buzau population, Lazarea population, Patraque d'Auvergne, RFA Roclas Clone 2.6 Ferma, Vitelotte Negresse, Roclas Clone C, Blue Congo) were measured with 532 nm laser line. Main surface-enhanced Raman modes of these DNAs have been analyzed. Also, DNA from two grapevine (Vitis vinifera L.) varieties were studied at acidic pHs by surface-enhanced Raman spectroscopy. Modified SERS intensities and wavenumber shifts of nucleic acids bands were observed upon lowering the pH, being a proof of binding affinity changes of DNA with silver nanoparticles (AgNPs) and of structural modifications induced at acidic pHs in DNA molecular groups. Furthermore, the (sub)picosecond surface dynamics of DNA extracted from leaf tissues of grapevine (Vitis vinifera L.) varieties was investigated. In this work, the bands full widths at half-maximum (FWHMs) have values in the wavenumber range from 8 to 34 cm-1. (Sub)picosecond molecular dynamics of DNA groups with global relaxation times between 0.31 ps - 1.33 ps has been found.
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MEDLINE
Asunto principal:
Ácidos Nucleicos
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Nanopartículas del Metal
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En
Año:
2022
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Article