Your browser doesn't support javascript.
loading
Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta).
Smedley, Jeremy V; Bochart, Rachele M; Fischer, Miranda; Funderburgh, Heidi; Kelly, Vanessa; Crank, Hugh; Armantrout, Kim; Shiel, Oriene; Robertson-LeVay, Mitchell; Sternberger, Nikki; Schmaling, Brian; Roberts, Sheila; Sekiguchi, Vicki; Reusz, Michael; Schwartz, Tiah; Meyer, Kimberly A; Webb, Gabriela; Gilbride, Roxanne M; Dambrauskas, Nicholas; Andrade, Daniela; Wood, Matthew; Labriola, Caralyn; Axthelm, Michael; Derby, Nina; Varco-Merth, Ben; Fukazawa, Yoshinori; Hansen, Scott; Sacha, Jonah B; Sodora, Donald L; Sather, D Noah.
  • Smedley JV; Infectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Bochart RM; Infectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Fischer M; Infectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Funderburgh H; Infectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Kelly V; Infectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Crank H; Infectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Armantrout K; Infectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Shiel O; Infectious Disease Resource, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Robertson-LeVay M; Surgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Sternberger N; Surgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Schmaling B; Surgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Roberts S; Surgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Sekiguchi V; Surgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Reusz M; Surgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Schwartz T; Surgical Services Unit, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Meyer KA; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
  • Webb G; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Gilbride RM; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Dambrauskas N; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
  • Andrade D; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
  • Wood M; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
  • Labriola C; Experimental Pathology Unit, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Axthelm M; Experimental Pathology Unit, Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Derby N; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
  • Varco-Merth B; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Fukazawa Y; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Hansen S; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Sacha JB; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USA.
  • Sodora DL; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
  • Sather DN; Department of Pediatrics, University of Washington, Seattle, Washington, USA.
J Med Primatol ; 51(5): 270-277, 2022 10.
Article en En | MEDLINE | ID: mdl-35841132
ABSTRACT

BACKGROUND:

Identification of lymph nodes (LNs) draining a specific site or in obese macaques can be challenging.

METHODS:

Indocyanine Green (ICG) was administered intradermal (ID), intramuscular, in the oral mucosa, or subserosal in the colon followed by Near Infrared (NIR) imaging.

RESULTS:

After optimization to maximize LN identification, intradermal ICG was successful in identifying 50-100% of the axillary/inguinal LN at a site. Using NIR, collection of peripheral and mesenteric LNs in obese macaques was 100% successful after traditional methods failed. Additionally, guided collection of LNs draining the site of intraepithelial or intramuscular immunization demonstrated significantly increased numbers of T follicular helper (Tfh) cells in germinal centers of draining compared to nondraining LNs.

CONCLUSION:

These imaging techniques optimize our ability to evaluate immune changes within LNs over time, even in obese macaques. This approach allows for targeted serial biopsies that permit confidence that draining LNs are being harvested throughout the study.
Asunto(s)
Palabras clave
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Verde de Indocianina / Ganglios Linfáticos Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Verde de Indocianina / Ganglios Linfáticos Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article