Fluorescence fluctuation-based super-resolution microscopy: Basic concepts for an easy start.
J Microsc
; 288(3): 218-241, 2022 12.
Article
en En
| MEDLINE
| ID: mdl-35896096
RESUMEN
Due to light's wave nature, an optical microscope's resolution range is 200 to 350 nanometers. Several techniques enhance resolution; this work encompasses several fluorescence fluctuation super-resolution (FF-SMR) methods capable of achieving nanoscopic scales. FF-SRM is known to be suitable for fixed or live-cell imaging and compatible with most conventional microscope setups found in a laboratory. However, each FF-SRM approach has its strengths and weaknesses, which depend directly on the underlying principles through which enhanced spatial resolution is achieved. Therefore, the basic concepts and principles behind diverse FF-SRM methods are revisited in this review. In addition, their operational parameters are explained, and guidance for their selection is provided for microscopists interested in FF-SRM.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Procesamiento de Imagen Asistido por Computador
/
Colorantes Fluorescentes
Idioma:
En
Año:
2022
Tipo del documento:
Article