Research strategies for single-cell transcriptome analysis in plant leaves.
Plant J
; 112(1): 27-37, 2022 10.
Article
en En
| MEDLINE
| ID: mdl-35904970
ABSTRACT
The recent and continuous improvement in single-cell RNA sequencing (scRNA-seq) technology has led to its emergence as an efficient experimental approach in plant research. However, compared with single-cell research in animals and humans, the application of scRNA-seq in plant research is limited by several challenges, including cell separation, cell type annotation, cellular function analysis, and cell-cell communication networks. In addition, the unavailability of corresponding reliable and stable analysis methods and standards has resulted in the relative decentralization of plant single-cell research. Considering these shortcomings, this review summarizes the research progress in plant leaf using scRNA-seq. In addition, it describes the corresponding feasible analytical methods and associated difficulties and problems encountered in the current research. In the end, we provide a speculative overview of the development of plant single-cell transcriptome research in the future.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Análisis de la Célula Individual
/
Transcriptoma
Límite:
Animals
/
Humans
Idioma:
En
Año:
2022
Tipo del documento:
Article