Biochemical, structural, and computational studies of a Î³-carbonic anhydrase from the pathogenic bacterium <i>Burkholderia pseudomallei</i>.

Biochemical, structural, and computational studies of a Î³-carbonic anhydrase from the pathogenic bacterium Burkholderia pseudomallei.

Di Fiore, Anna; De Luca, Viviana; Langella, Emma; Nocentini, Alessio; Buonanno, Martina; Monti, Simona Maria; Supuran, Claudiu T; Capasso, Clemente; De Simone, Giuseppina.

Di Fiore A; Institute of Biostructures and Bioimaging-CNR, via Pietro Castellino 111, 80131 Napoli, Italy.
De Luca V; Institute of Biosciences and Bioresources-CNR, via Pietro Castellino 111, 80131 Napoli, Italy.
Langella E; Institute of Biostructures and Bioimaging-CNR, via Pietro Castellino 111, 80131 Napoli, Italy.
Nocentini A; NEUROFARBA Department, Pharmaceutical and Nutraceutical Section, University of Firenze, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Italy.
Buonanno M; Institute of Biostructures and Bioimaging-CNR, via Pietro Castellino 111, 80131 Napoli, Italy.
Monti SM; Institute of Biostructures and Bioimaging-CNR, via Pietro Castellino 111, 80131 Napoli, Italy.
Supuran CT; NEUROFARBA Department, Pharmaceutical and Nutraceutical Section, University of Firenze, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Italy.
Capasso C; Institute of Biosciences and Bioresources-CNR, via Pietro Castellino 111, 80131 Napoli, Italy.
De Simone G; Institute of Biostructures and Bioimaging-CNR, via Pietro Castellino 111, 80131 Napoli, Italy.

Comput Struct Biotechnol J ; 20: 4185-4194, 2022.

Article en En | MEDLINE | ID: mdl-36016712

Palabras clave

BME, 2-betamercaptoethanol; BSA, Bovine Serum Albumin; BpsßCA, ß-CA from B. pseudomallei; BpsÎ³CA, Î³-CA from B. pseudomallei; Burkholderia pseudomallei; CA, Carbonic Anhydrase; CA_D, Î³-CA from Discovery Deep Brine Pool; CCD, Charge Coupled Device; CD, circular dichroism; Cag, Î³-CA from G. kaustophilus; Cam, Î³-CA from M. thermophila; Carbonic anhydrases; Crystal structure; Cyt C, horse Cytochrome C; Melioidosis; PDB, Protein Data Bank; PEG, Polyethylene glycol; PSR, proton shuttle residue; RicA, Î³-CA from B. abortus; SEC, size exclusion chromatography; TeCcmM, Î³-CA from T. elongatus; TtCA, Î³-CA from T. thermophilus HB8; Yrda, Î³-CA from E. coli; Zn-Cap, Î³-CA from P. horikoshii; pKa calculations; r.s.m.d., root mean square deviation

Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2022 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2022 Tipo del documento: Article