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Systemic lipolysis promotes physiological fitness in Drosophila melanogaster.
Shang, Linshan; Aughey, Elizabeth; Kim, Huiseon; Heden, Timothy D; Wang, Lu; Najt, Charles P; Esch, Nicholas; Brunko, Sophia; Abrahante, Juan E; Macchietto, Marissa; Mashek, Mara T; Fairbanks, Todd; Promislow, Daniel E L; Neufeld, Thomas P; Mashek, Douglas G.
  • Shang L; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
  • Aughey E; Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA.
  • Kim H; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
  • Heden TD; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
  • Wang L; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA 98105, USA.
  • Najt CP; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
  • Esch N; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
  • Brunko S; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
  • Abrahante JE; University of Minnesota Informatics Institute, Minneapolis, MN 55455, USA.
  • Macchietto M; Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN 55455, USA.
  • Mashek MT; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
  • Fairbanks T; Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA.
  • Promislow DEL; Department of Biology, University of Washington, Seattle, WA 98195, USA.
  • Neufeld TP; Department of Lab Medicine and Pathology, University of Washington School of Medicine, Seattle, WA 98195, USA.
  • Mashek DG; Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA.
Aging (Albany NY) ; 14(16): 6481-6506, 2022 Aug 30.
Article en En | MEDLINE | ID: mdl-36044277
Since interventions such as caloric restriction or fasting robustly promote lipid catabolism and improve aging-related phenotypical markers, we investigated the direct effect of increased lipid catabolism via overexpression of bmm (brummer, FBgn0036449), the major triglyceride hydrolase in Drosophila, on lifespan and physiological fitness. Comprehensive characterization was carried out using RNA-seq, lipidomics and metabolomics analysis. Global overexpression of bmm strongly promoted numerous markers of physiological fitness, including increased female fecundity, fertility maintenance, preserved locomotion activity, increased mitochondrial biogenesis and oxidative metabolism. Increased bmm robustly upregulated the heat shock protein 70 (Hsp70) family of proteins, which equipped the flies with higher resistance to heat, cold, and ER stress via improved proteostasis. Despite improved physiological fitness, bmm overexpression did not extend lifespan. Taken together, these data show that bmm overexpression has broad beneficial effects on physiological fitness, but these effects did not impact lifespan.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas de Drosophila / Drosophila melanogaster Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas de Drosophila / Drosophila melanogaster Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article