Arthropod-Borne Virus Surveillance as a Tool to Study the Australian Mosquito Virome.
Viruses
; 14(9)2022 08 26.
Article
en En
| MEDLINE
| ID: mdl-36146689
ABSTRACT
Mosquitoes (n = 4381 in 198 pools) were collected in March and April 2018 to survey the presence of West Nile virus Kunjin strain in mosquito populations around crocodile farms in the Darwin region of the Northern Territory (NT) of Australia. While no Kunjin virus was detected in these mosquitoes, we applied our viral replicative intermediates screening system termed monoclonal antibodies to viral RNA intermediates in cells or MAVRIC to this set of samples. This resulted in the detection of 28 pools with virus replicating in C6/36 mosquito cells and the identification of three insect viruses from three distinct virus classes. We demonstrate the persistence of the insect-specific flavivirus Palm Creek virus in Coquillettidia xanthogaster mosquitoes from Darwin over almost a decade, with limited genetic drift. We also detected a novel Hubei macula-like virus 3 strain in samples from two mosquito genera, suggesting the virus, for which the sequence was originally detected in spiders and soybean thrips, might be involved in a horizontal transmission cycle between arthropods and plants. Overall, these data demonstrate the strength of the optimized MAVRIC system and contribute to our general knowledge of the mosquito virome and insect viruses.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Arbovirus
/
Virus del Nilo Occidental
/
Flavivirus
/
Virus de Insectos
/
Culicidae
Tipo de estudio:
Screening_studies
Límite:
Animals
País como asunto:
Oceania
Idioma:
En
Año:
2022
Tipo del documento:
Article