A synthetic C2 auxotroph of Pseudomonas putida for evolutionary engineering of alternative sugar catabolic routes.

Wirth, Nicolas T; Gurdo, Nicolás; Krink, Nicolas; Vidal-Verdú, Àngela; Donati, Stefano; Férnandez-Cabezón, Lorena; Wulff, Tune; Nikel, Pablo I

Wirth, Nicolas T; Gurdo, Nicolás; Krink, Nicolas; Vidal-Verdú, Àngela; Donati, Stefano; Férnandez-Cabezón, Lorena; Wulff, Tune; Nikel, Pablo I.

Wirth NT; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220 2800, Kongens Lyngby, Denmark.
Gurdo N; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220 2800, Kongens Lyngby, Denmark.
Krink N; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220 2800, Kongens Lyngby, Denmark.
Vidal-Verdú À; Institute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Calle del Catedràtic Agustin Escardino Benlloch 9, 46980, Paterna, Spain.
Donati S; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220 2800, Kongens Lyngby, Denmark.
Férnandez-Cabezón L; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220 2800, Kongens Lyngby, Denmark.
Wulff T; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220 2800, Kongens Lyngby, Denmark.
Nikel PI; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220 2800, Kongens Lyngby, Denmark. Electronic address: pabnik@biosustain.dtu.dk.

Metab Eng ; 74: 83-97, 2022 11.

Article en En | MEDLINE | ID: mdl-36155822

Asunto(s)

Pseudomonas putida; Pseudomonas putida/genética; Pseudomonas putida/metabolismo; Azúcares/metabolismo; Análisis de Flujos Metabólicos; Redes y Vías Metabólicas/genética; Glucosa/genética; Glucosa/metabolismo; Ingeniería Metabólica

Palabras clave

Adaptive laboratory evolution; Metabolic engineering; Phosphoketolase; Pseudomonas putida; Synthetic auxotrophy; Synthetic biology; Synthetic metabolism

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Pseudomonas putida Idioma: En Año: 2022 Tipo del documento: Article

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Pseudomonas putida Idioma: En Año: 2022 Tipo del documento: Article