Your browser doesn't support javascript.
loading
Detection of intestinal parasites in human faecal samples using dissolved air flotation.
Soares, Felipe Augusto; Dos Santos, Bianca Martins; Rosa, Stefany Laryssa; Loiola, Saulo Hudson Nery; Stéfano, Vitória Castilho; Proença, Letícia Rodrigues; Suzuki, Celso Tetsuo Nagase; Sabadini, Edvaldo; Bresciani, Katia Denise Saraiva; Falcão, Alexandre Xavier; Gomes, Jancarlo Ferreira.
  • Soares FA; School of Medical Sciences, University of Campinas, Campinas, São Paulo, Brazil.
  • Dos Santos BM; Institute of Computing (IC), Laboratory of Image Data Science (LIDS), University of Campinas, Campinas, São Paulo, Brazil.
  • Rosa SL; School of Medical Sciences, University of Campinas, Campinas, São Paulo, Brazil.
  • Loiola SHN; Institute of Computing (IC), Laboratory of Image Data Science (LIDS), University of Campinas, Campinas, São Paulo, Brazil.
  • Stéfano VC; School of Medical Sciences, University of Campinas, Campinas, São Paulo, Brazil.
  • Proença LR; Institute of Computing (IC), Laboratory of Image Data Science (LIDS), University of Campinas, Campinas, São Paulo, Brazil.
  • Suzuki CTN; School of Medical Sciences, University of Campinas, Campinas, São Paulo, Brazil.
  • Sabadini E; Institute of Computing (IC), Laboratory of Image Data Science (LIDS), University of Campinas, Campinas, São Paulo, Brazil.
  • Bresciani KDS; Institute of Computing (IC), Laboratory of Image Data Science (LIDS), University of Campinas, Campinas, São Paulo, Brazil.
  • Falcão AX; Institute of Computing (IC), Laboratory of Image Data Science (LIDS), University of Campinas, Campinas, São Paulo, Brazil.
  • Gomes JF; Institute of Computing (IC), Laboratory of Image Data Science (LIDS), University of Campinas, Campinas, São Paulo, Brazil.
Trop Med Int Health ; 27(12): 1044-1052, 2022 12.
Article en En | MEDLINE | ID: mdl-36261920
ABSTRACT

OBJECTIVE:

Ova and parasite (O&P) examination is recommended for the laboratory diagnosis of agents causing parasitic infections; however, this exam requires scientific and technological improvements to enhance its diagnostic validity. Dissolved air flotation (DAF) is an efficient technical principle separating suspended solids in a liquid medium. We aimed to develop and validate a new procedure for intestinal parasite detection with DAF.

METHODS:

In this study, we collected samples from 500 volunteers, screened them by direct examination, and transferred the material to tubes using the Three Faecal Test (TF-Test) for triplicate DAF tests. We evaluated physical-chemical parameters and DAF prototype components through quantifying parasites recovered from floated and non-floated regions of the flotation column. The DAF operation protocol was validated with the gold standard results.

RESULTS:

The 10% saturated volume proportion and cationic surfactant showed regularity and high parasite recovery (80%). Modifications of the needle device did not influence parasite recovery (p > 0.05). Sensitivity, specificity, accuracy and kappa agreement obtained with the DAF protocol were 91%, 100%, 93% and substantial (k = 0.64), respectively.

CONCLUSION:

The DAF principle could be used to process faecal samples in routine laboratory exams, enabling intestinal parasite detection.
Asunto(s)
Palabras clave
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Parásitos / Parasitosis Intestinales Tipo de estudio: Diagnostic_studies / Guideline Límite: Animals / Humans Idioma: En Año: 2022 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Parásitos / Parasitosis Intestinales Tipo de estudio: Diagnostic_studies / Guideline Límite: Animals / Humans Idioma: En Año: 2022 Tipo del documento: Article