A gene chip study suggests that miR-17-3p is associated with diabetic foot ulcers.
Int Wound J
; 20(5): 1525-1533, 2023 May.
Article
en En
| MEDLINE
| ID: mdl-36333728
ABSTRACT
Background of the Study Diabetic foot ulcers (DFUs) are severe effect of diabetes. This research aimed to discover the role of micro-ribonucleic acid (miRNA) in treating DFUs involved in maggot debridement therapy (MDT) via a miRNA chip study. A miRNA chip approach was adopted. Patients with diabetes (type 1 or 2) who had at least one-foot ulcer (current or previous) were enrolled in the study. The alterations of miRNA expressions in the granulation tissue during treatment with MDT were measured. Following MDT, the increased expression of miR17-92 was verified in vivo. The miR-17-3p expression increased, and Flk-1 (vascular endothelial growth factor) expression was significantly reduced in patients with DFUs who received MDT (P < 0.01). Results from human umbilical vein endothelial cells that excrete or secrete showed consistency with in vitro findings (P < 0.001, P < 0.05). The overexpression of miR-17-3p demonstrated inhibitory activity on tube formation (P < 0.05). When DFUs were treated with MDT, it revealed that miR-17-3p had a negative regulatory effect on Flk-1.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pie Diabético
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MicroARNs
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Diabetes Mellitus
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
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Humans
Idioma:
En
Año:
2023
Tipo del documento:
Article