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Modeling Alzheimer's Disease Using Human Brain Organoids.
Karmirian, Karina; Holubiec, Mariana; Goto-Silva, Livia; Fernandez Bessone, Ivan; Vitória, Gabriela; Mello, Beatriz; Alloatti, Matias; Vanderborght, Bart; Falzone, Tomás L; Rehen, Stevens.
  • Karmirian K; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil.
  • Holubiec M; Institute of Biomedical Sciences, Rio de Janeiro, Brazil.
  • Goto-Silva L; Instituto de Biología Celular y Neurociencia IBCN (UBA-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Fernandez Bessone I; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil.
  • Vitória G; Instituto de Biología Celular y Neurociencia IBCN (UBA-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Mello B; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil.
  • Alloatti M; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil.
  • Vanderborght B; Instituto de Biología Celular y Neurociencia IBCN (UBA-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Falzone TL; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil.
  • Rehen S; Instituto de Biología Celular y Neurociencia IBCN (UBA-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
Methods Mol Biol ; 2561: 135-158, 2023.
Article en En | MEDLINE | ID: mdl-36399268
ABSTRACT
Alzheimer's disease (AD) is the primary cause of dementia, to date. The urgent need to understand the biological and biochemical processes related to this condition, as well as the demand for reliable in vitro models for drug screening, has led to the development of novel techniques, among which stem cell methods are of utmost relevance for AD research, particularly the development of human brain organoids. Brain organoids are three-dimensional cellular aggregates derived from induced pluripotent stem cells (iPSCs) that recreate different neural cell interactions and tissue characteristics in culture. Here, we describe the protocol for the generation of brain organoids derived from AD patients and for the analysis of AD-derived pathology. AD organoids can recapitulate beta-amyloid and tau pathological features, making them a promising model for studying the molecular mechanisms underlying disease and for in vitro drug testing.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas / Enfermedad de Alzheimer Límite: Humans Idioma: En Año: 2023 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas / Enfermedad de Alzheimer Límite: Humans Idioma: En Año: 2023 Tipo del documento: Article