A fluorogenic probe for core-fucosylated glycan-preferred ENGase.

Ishii, Nozomi; Muto, Hiroshi; Nagata, Mitsuo; Sano, Kanae; Sato, Itsuki; Iino, Kenta; Matsuzaki, Yuji; Katoh, Toshihiko; Yamamoto, Kenji; Matsuo, Ichiro

Ishii, Nozomi; Muto, Hiroshi; Nagata, Mitsuo; Sano, Kanae; Sato, Itsuki; Iino, Kenta; Matsuzaki, Yuji; Katoh, Toshihiko; Yamamoto, Kenji; Matsuo, Ichiro.

Ishii N; Graduate School of Science and Technology, Gunma University, 1-5-1, Tenjin-cho, Kiryu, Gunma, 376-8515, Japan.
Muto H; Graduate School of Science and Technology, Gunma University, 1-5-1, Tenjin-cho, Kiryu, Gunma, 376-8515, Japan; Biologics Technology Research Laboratories, Daiichi Sankyo Co., Ltd., 3-5-1, Nihonbashi-honcho, Tokyo, 103-8426, Japan.
Nagata M; Graduate School of Science and Technology, Gunma University, 1-5-1, Tenjin-cho, Kiryu, Gunma, 376-8515, Japan.
Sano K; Graduate School of Science and Technology, Gunma University, 1-5-1, Tenjin-cho, Kiryu, Gunma, 376-8515, Japan.
Sato I; Graduate School of Science and Technology, Gunma University, 1-5-1, Tenjin-cho, Kiryu, Gunma, 376-8515, Japan.
Iino K; Glyco Synthetic Lab., Tokyo Chemical Industry Co., Ltd, 6-15-9 Toshima, Kita-ku, Tokyo, 114-0003, Japan.
Matsuzaki Y; Glyco Synthetic Lab., Tokyo Chemical Industry Co., Ltd, 6-15-9 Toshima, Kita-ku, Tokyo, 114-0003, Japan.
Katoh T; Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan.
Yamamoto K; Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan.
Matsuo I; Graduate School of Science and Technology, Gunma University, 1-5-1, Tenjin-cho, Kiryu, Gunma, 376-8515, Japan. Electronic address: matsuo@gunma-u.ac.jp.

Carbohydr Res ; 523: 108724, 2023 Jan.

Article en En | MEDLINE | ID: mdl-36435009

Asunto(s)

Polisacáridos; Polisacáridos/química; Glicosilación; Manosil-Glicoproteína Endo-beta-N-Acetilglucosaminidasa/química; Manosil-Glicoproteína Endo-beta-N-Acetilglucosaminidasa/metabolismo; Especificidad por Sustrato

Palabras clave

Chemical probe; Core-fucosylated N-Glycan; Endo-glycosidase; Endo-ß-N-Acetylglucosaminidase; FRET quenching

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