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Zebrafish as a potential model for stroke: A comparative study with standardized models.
de Medeiros Borges, Heloisa; Dagostin, Caroline Serafim; Córneo, Emily; Dondossola, Eduardo Ronconi; Bernardo, Henrique Teza; Pickler, Karolyne De Pieri; da Costa Pereira, Bárbara; de Oliveira, Mariane Amanda; Scussel, Rahisa; Michels, Monique; Machado-de-Ávila, Ricardo Andrez; Dal-Pizzol, Felipe; Rico, Eduardo Pacheco.
  • de Medeiros Borges H; Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, Brazil; Translational Psychiatry Laboratory, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, SC, Brazil.
  • Dagostin CS; Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, Brazil.
  • Córneo E; Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, Brazil.
  • Dondossola ER; Translational Psychiatry Laboratory, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, SC, Brazil.
  • Bernardo HT; Translational Psychiatry Laboratory, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, SC, Brazil.
  • Pickler KP; Translational Psychiatry Laboratory, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, SC, Brazil.
  • da Costa Pereira B; Translational Psychiatry Laboratory, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, SC, Brazil.
  • de Oliveira MA; Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, Brazil.
  • Scussel R; Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, Brazil.
  • Michels M; Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, Brazil; Gabbia Biotechnology Company, Barra Velha, Santa Catarina, Brazil.
  • Machado-de-Ávila RA; Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, Brazil.
  • Dal-Pizzol F; Laboratory of Experimental Pathophysiology, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, Brazil.
  • Rico EP; Translational Psychiatry Laboratory, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, SC, Brazil. Electronic address: eduprico@gmail.com.
Life Sci ; 312: 121200, 2023 Jan 01.
Article en En | MEDLINE | ID: mdl-36435227
ABSTRACT
Animal models of cerebral ischemia have improved our understanding of the pathophysiology and mechanisms involved in stroke, as well as the investigation of potential therapies. The potential of zebrafish to model human diseases has become increasingly evident. The availability of these models allows for an increased understanding of the role of chemical exposure in human conditions and provides essential tools for mechanistic studies of disease. To evaluate the potential neuroprotective properties of minocycline against ischemia and reperfusion injury in zebrafish and compare them with other standardized models. In vitro studies with BV-2 cells were performed, and mammalian transient middle cerebral artery occlusion (tMCAO) was used as a comparative standard with the zebrafish stroke model. Animals were subjected to ischemia and reperfusion injury protocols and treated with minocycline. Infarction size, cytokine levels, oxidative stress, glutamate toxicity, and immunofluorescence for microglial activation, and behavioral test results were determined and compared. Administration of minocycline provided significant protection in the three stroke models in different parameters analyzed. Both experimental models complement each other in their particularities. The proposal also strengthens the findings in the literature in rodent models and allows the validation of alternative models so that they can be used in further research involving diseases with ischemia and reperfusion injury.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Daño por Reperfusión / Isquemia Encefálica / Fármacos Neuroprotectores / Accidente Cerebrovascular Tipo de estudio: Guideline / Prognostic_studies Límite: Animals / Humans Idioma: En Año: 2023 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Daño por Reperfusión / Isquemia Encefálica / Fármacos Neuroprotectores / Accidente Cerebrovascular Tipo de estudio: Guideline / Prognostic_studies Límite: Animals / Humans Idioma: En Año: 2023 Tipo del documento: Article