Your browser doesn't support javascript.
loading
Engineered cell entry links receptor biology with single-cell genomics.
Yu, Bingfei; Shi, Quanming; Belk, Julia A; Yost, Kathryn E; Parker, Kevin R; Li, Rui; Liu, Betty B; Huang, Huang; Lingwood, Daniel; Greenleaf, William J; Davis, Mark M; Satpathy, Ansuman T; Chang, Howard Y.
  • Yu B; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA.
  • Shi Q; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Belk JA; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Yost KE; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA.
  • Parker KR; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA.
  • Li R; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA.
  • Liu BB; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
  • Huang H; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA; Institute for Immunity, Transplantation and Infection, Stanford University, Stanford, CA, USA.
  • Lingwood D; The Ragon Institute of Massachusetts General Hospital, The Massachusetts Institute of Technology and Harvard University, Cambridge, MA, USA.
  • Greenleaf WJ; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
  • Davis MM; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA; Institute for Immunity, Transplantation and Infection, Stanford University, Stanford, CA, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Satpathy AT; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Chang HY; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: howchang@stanford.edu.
Cell ; 185(26): 4904-4920.e22, 2022 12 22.
Article en En | MEDLINE | ID: mdl-36516854
ABSTRACT
Cells communicate with each other via receptor-ligand interactions. Here, we describe lentiviral-mediated cell entry by engineered receptor-ligand interaction (ENTER) to display ligand proteins, deliver payloads, and record receptor specificity. We optimize ENTER to decode interactions between T cell receptor (TCR)-MHC peptides, antibody-antigen, and other receptor-ligand pairs. A viral presentation strategy allows ENTER to capture interactions between B cell receptor and any antigen. We engineer ENTER to deliver genetic payloads to antigen-specific T or B cells to selectively modulate cellular behavior in mixed populations. Single-cell readout of ENTER by RNA sequencing (ENTER-seq) enables multiplexed enumeration of antigen specificities, TCR clonality, cell type, and states of individual T cells. ENTER-seq of CMV-seropositive patient blood samples reveals the viral epitopes that drive effector memory T cell differentiation and inter-clonal vs. intra-clonal phenotypic diversity targeting the same epitope. ENTER technology enables systematic discovery of receptor specificity, linkage to cell fates, and antigen-specific cargo delivery.
Asunto(s)
Palabras clave
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Receptores de Antígenos de Linfocitos T / Internalización del Virus Límite: Humans Idioma: En Año: 2022 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Receptores de Antígenos de Linfocitos T / Internalización del Virus Límite: Humans Idioma: En Año: 2022 Tipo del documento: Article