Effects of aerobic exercise training in oxidative metabolism and mitochondrial biogenesis markers on prefrontal cortex in obese mice.

de Sousa Fernandes, Matheus Santos; Aidar, Felipe J; da Silva Pedroza, Anderson Apolônio; de Andrade Silva, Severina Cássia; Santos, Gabriela Carvalho Jurema; Dos Santos Henrique, Rafael; Clemente, Filipe Manuel; Silva, Ana Filipa; de Souza, Raphael Fabrício; Ferreira, Diorginis José; Badicu, Georgian; Lagranha, Claudia; Nobari, Hadi

de Sousa Fernandes, Matheus Santos; Aidar, Felipe J; da Silva Pedroza, Anderson Apolônio; de Andrade Silva, Severina Cássia; Santos, Gabriela Carvalho Jurema; Dos Santos Henrique, Rafael; Clemente, Filipe Manuel; Silva, Ana Filipa; de Souza, Raphael Fabrício; Ferreira, Diorginis José; Badicu, Georgian; Lagranha, Claudia; Nobari, Hadi.

de Sousa Fernandes MS; Graduate Program in Neuropsychiatry and Behavioral Sciences, Center for Medical Sciences, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
Aidar FJ; Department of Physical Education, Federal University of Sergipe, São Cristovão, Sergipe, Brazil.
da Silva Pedroza AA; Laboratory of Biochemistry and Exercise Biochemistry, Department of Physical Education and Sports Science, Federal University of Pernambuco, Vitória de Santo Antão, PE, Brazil.
de Andrade Silva SC; Laboratory of Biochemistry and Exercise Biochemistry, Department of Physical Education and Sports Science, Federal University of Pernambuco, Vitória de Santo Antão, PE, Brazil.
Santos GCJ; Department of Nutrition, Federal University of Pernambuco, Recife, Brazil.
Dos Santos Henrique R; Department of Physical Education, Federal University of Pernambuco, Recife, Brazil.
Clemente FM; Escola Superior Desporto e Lazer, Instituto Politécnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun'Álvares, 4900-347, Viana do Castelo, Portugal.
Silva AF; Instituto de Telecomunicações, Delegação da Covilhã, 1049-001, Lisbon, Portugal.
de Souza RF; Research Center in Sports Performance, Recreation, Innovation and Technology (SPRINT), 4960-320, Melgaço, Portugal.
Ferreira DJ; Escola Superior Desporto e Lazer, Instituto Politécnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun'Álvares, 4900-347, Viana do Castelo, Portugal.
Badicu G; Research Center in Sports Performance, Recreation, Innovation and Technology (SPRINT), 4960-320, Melgaço, Portugal.
Lagranha C; Department of Physical Education, Federal University of Sergipe, São Cristovão, Sergipe, Brazil.
Nobari H; Department of Physical Education, Federal University of São Francisco Valley, Petrolina, Pernambuco, Brazil.

BMC Sports Sci Med Rehabil ; 14(1): 213, 2022 Dec 16.

Article en En | MEDLINE | ID: mdl-36527152

ABSTRACT

ABSTRACT

BACKGROUND:

To evaluate the effects of 8 weeks of Aerobic Physical Training (AET) on the mitochondrial biogenesis and oxidative balance in the Prefrontal Cortex (PFC) of leptin deficiency-induced obese mice (ob/ob mice).

METHODS:

Then, the mice were submitted to an 8-week protocol of aerobic physical training (AET) at moderate intensity (60% of the maximum running speed). In the oxidative stress, we analyzed Malonaldehyde (MDA) and Carbonyls, the enzymatic activity of Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione S Transferase (GST), non-enzymatic antioxidant system reduced glutathione (GSH), and Total thiols. Additionally, we evaluated the gene expression of PGC-1α SIRT-1, and ATP5A related to mitochondrial biogenesis and function.

RESULTS:

In our study, we did not observe a significant difference in MDA (p = 0.2855), Carbonyl's (p = 0.2246), SOD (p = 0.1595), and CAT (p = 0.6882) activity. However, the activity of GST (p = 0.04), the levels of GSH (p = 0.001), and Thiols (p = 0.02) were increased after 8 weeks of AET. Additionally, there were high levels of PGC-1α (p = 0.01), SIRT-1 (p = 0.009), and ATP5A (p = 0.01) gene expression after AET in comparison with the sedentary group.