A flow-cytometry-based assay to assess granule exocytosis and GZB delivery by human CD8 T cells and NK cells.
STAR Protoc
; 4(1): 101939, 2023 03 17.
Article
en En
| MEDLINE
| ID: mdl-36527713
ABSTRACT
CD8 T and NK cells mediate killing by delivery of perforin and granzyme B (GZB) stored in lysosome-like granules. We present a flow-cytometry-based protocol combined with a redirected killing assay to evaluate granule exocytosis and the cytotoxic potential of human CD8 T cells and NK cells. We describe the assessment of the delivered GZB inside the target cells. We then detail the detection of lysosome membrane protein CD107a exposed on the cell surface of the effector cells upon degranulation. For complete details on the use and execution of this protocol, please refer to Chen et al. (2021).1.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Asesinas Naturales
/
Linfocitos T CD8-positivos
Límite:
Humans
Idioma:
En
Año:
2023
Tipo del documento:
Article