A simple and effective method to purify and activate T cells for successful generation of chimeric antigen receptor T (CAR-T) cells from patients with high monocyte count.

Wang, Haiying; Tsao, Shih-Ting; Gu, Mingyuan; Fu, Chengbing; He, Feng; Li, Xiu; Zhang, Mian; Li, Na; Hu, Hong-Ming

Wang, Haiying; Tsao, Shih-Ting; Gu, Mingyuan; Fu, Chengbing; He, Feng; Li, Xiu; Zhang, Mian; Li, Na; Hu, Hong-Ming.

Wang H; Department of Research and Development, Hrain Biotechnology Co., Ltd., 9th Floor, Building 1, 1238 Zhangjiang Road, Pudong New District, Shanghai, China.
Tsao ST; Department of Research and Development, Hrain Biotechnology Co., Ltd., 9th Floor, Building 1, 1238 Zhangjiang Road, Pudong New District, Shanghai, China.
Gu M; Department of Research and Development, Hrain Biotechnology Co., Ltd., 9th Floor, Building 1, 1238 Zhangjiang Road, Pudong New District, Shanghai, China.
Fu C; Department of Research and Development, Hrain Biotechnology Co., Ltd., 9th Floor, Building 1, 1238 Zhangjiang Road, Pudong New District, Shanghai, China.
He F; Department of Manufacturing, Hrain Biotechnology Co., Ltd., 9th Floor, Building 1, 1238 Zhangjiang Road, Pudong New District, Shanghai, China.
Li X; Department of Research and Development, Hrain Biotechnology Co., Ltd., 9th Floor, Building 1, 1238 Zhangjiang Road, Pudong New District, Shanghai, China.
Zhang M; Department of Research and Development, Hrain Biotechnology Co., Ltd., 9th Floor, Building 1, 1238 Zhangjiang Road, Pudong New District, Shanghai, China.
Li N; Department of Research and Development, Hrain Biotechnology Co., Ltd., 9th Floor, Building 1, 1238 Zhangjiang Road, Pudong New District, Shanghai, China.
Hu HM; Department of Research and Development, Hrain Biotechnology Co., Ltd., 9th Floor, Building 1, 1238 Zhangjiang Road, Pudong New District, Shanghai, China. hhu004@gmail.com.

J Transl Med ; 20(1): 608, 2022 12 19.

Article en En | MEDLINE | ID: mdl-36536403

Asunto(s)

Linfoma; Receptores Quiméricos de Antígenos; Humanos; Antígenos CD19; Antígeno de Maduración de Linfocitos B; Inmunoterapia Adoptiva/métodos; Leucocitos Mononucleares; Monocitos; Receptores de Antígenos de Linfocitos T; Linfocitos T

Palabras clave

Chimeric antigen receptor T (CAR-T) cells; Design of experiments (DoE); Monocyte; T-cell activation; T-cell purification

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Receptores Quiméricos de Antígenos / Linfoma Límite: Humans Idioma: En Año: 2022 Tipo del documento: Article

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