The Influence of Preanalytical Biospecimen Handling on the Measurement of B Vitamers, Amino Acids, and Other Metabolites in Blood.
Biopreserv Biobank
; 21(5): 467-476, 2023 Oct.
Article
en En
| MEDLINE
| ID: mdl-36622937
ABSTRACT
Introduction:
Sample handling can influence biomarker measurement and introduce variability when combining data from multiple studies or study sites. To inform the development of blood collection protocols within a multisite cohort study, we directly quantified concentrations of 54 biomarkers in blood samples subjected to different handling conditions. Materials andMethods:
We obtained serum, lithium heparin plasma, and EDTA plasma from 20 adult volunteers. Tubes of chilled whole blood were either centrifuged and processed within 2 hours of collection (the "reference standard") or were stored with cool packs for 24 or 48 hours; centrifuged before and/or after this delay; or collected in tubes with/without gel separators. We used linear mixed models with random intercepts to estimate geometric mean concentrations and relative percent differences across the conditions.Results:
Compared to the reference standard tubes, concentrations of many biomarkers changed after processing delays, but changes were often small. In serum, we observed large differences for B vitamers, glutamic acid (37% and 73% increases with 24- and 48-hour delays, respectively), glycine (12% and 23% increases), serine (16% and 27% increases), and acetoacetate (-19% and -26% decreases). Centrifugation timing and separator tube use did not affect concentrations of most biomarkers.Conclusion:
Sample handling should be consistent across samples within an analysis. The length of processing delays should be recorded and accounted for when this is not feasible.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Recolección de Muestras de Sangre
/
Aminoácidos
Tipo de estudio:
Observational_studies
Límite:
Adult
/
Humans
Idioma:
En
Año:
2023
Tipo del documento:
Article