Coloradocin, an antibiotic from a new Actinoplanes. II. Identity with luminamicin and elucidation of structure.
J Antibiot (Tokyo)
; 40(10): 1383-93, 1987 Oct.
Article
en En
| MEDLINE
| ID: mdl-3680004
ABSTRACT
Coloradocin was isolated from a fermentation broth by adsorption onto Amberlite XAD-2. The activity was eluted in MeOH and purified by gel filtration on Shephadex LH-20, followed by liquid-liquid chromatography on diol-bonded silica gel. The last two steps in the purification of this antibiotic included reverse-phase chromatography on C18-bonded silica gel and countercurrent chromatography on an Ito Coil Planet Centrifuge to give material of 90% purity. Analytically pure material was obtained by preparative HPLC. As a result of extensive homo and heteronuclear two-dimensional NMR studies, a structure was proposed for coloradocin. The structure consists of a decalin ring system fused to a 10-membered macrolactone which in turn is fused to a 14-membered macrolactone possessing an enol ether in conjugation with an unsaturated cyclic anhydride functionality. Coloradocin is related to a small class of antibiotics which include nodusmicin and nargenicin and was shown to be identical to luminamicin for which no structure has been reported.
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Banco de datos:
MEDLINE
Asunto principal:
Antibacterianos
/
Antibióticos Antineoplásicos
Idioma:
En
Año:
1987
Tipo del documento:
Article