Mutation of a distal gating residue modulates NADH binding in NADH:Quinone oxidoreductase from Pseudomonas aeruginosa PAO1.

Moni, Bilkis Mehrin; Quaye, Joanna A; Gadda, Giovanni

Moni, Bilkis Mehrin; Quaye, Joanna A; Gadda, Giovanni.

Moni BM; Department of Chemistry, Georgia State University, Atlanta, Georgia, USA.
Quaye JA; Department of Chemistry, Georgia State University, Atlanta, Georgia, USA.
Gadda G; Department of Chemistry, Georgia State University, Atlanta, Georgia, USA; Department of Biology, Georgia State University, Atlanta, Georgia, USA; The Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, Georgia, USA. Electronic address: ggadda@gsu.edu.

J Biol Chem ; 299(4): 103044, 2023 04.

Article en En | MEDLINE | ID: mdl-36803963

Asunto(s)

NAD(P)H Deshidrogenasa (Quinona); NAD; Pseudomonas aeruginosa; Flavinas/metabolismo; Cinética; Mutación; NAD/metabolismo; Oxidación-Reducción; Pseudomonas aeruginosa/enzimología; Pseudomonas aeruginosa/genética; NAD(P)H Deshidrogenasa (Quinona)/genética

Palabras clave

NADH:quinone oxidoreductase; binding affinity; gating residue; hydride transfer; reductive half-reaction; steady-state kinetics

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / NAD(P)H Deshidrogenasa (Quinona) / NAD Idioma: En Año: 2023 Tipo del documento: Article

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / NAD(P)H Deshidrogenasa (Quinona) / NAD Idioma: En Año: 2023 Tipo del documento: Article