Vertical growth dynamics of biofilms.
Proc Natl Acad Sci U S A
; 120(11): e2214211120, 2023 03 14.
Article
en En
| MEDLINE
| ID: mdl-36881625
ABSTRACT
During the biofilm life cycle, bacteria attach to a surface and then reproduce, forming crowded, growing communities. Many theoretical models of biofilm growth dynamics have been proposed; however, difficulties in accurately measuring biofilm height across relevant time and length scales have prevented testing these models, or their biophysical underpinnings, empirically. Using white light interferometry, we measure the heights of microbial colonies with nanometer precision from inoculation to their final equilibrium height, producing a detailed empirical characterization of vertical growth dynamics. We propose a heuristic model for vertical growth dynamics based on basic biophysical processes inside a biofilm diffusion and consumption of nutrients and growth and decay of the colony. This model captures the vertical growth dynamics from short to long time scales (10 min to 14 d) of diverse microorganisms, including bacteria and fungi.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Biopelículas
/
Heurística
Idioma:
En
Año:
2023
Tipo del documento:
Article