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Antibody induction in mice by liposome-displayed recombinant enterotoxigenic Escherichia coli (ETEC) colonization antigens.
Zhou, Shiqi; Yu, Karl O A; Mabrouk, Moustafa T; Jahagirdar, Dushyant; Huang, Wei-Chiao; Guerra, Julio A; He, Xuedan; Ortega, Joaquin; Poole, Steven T; Hall, Eric R; Gomez-Duarte, Oscar G; Maciel, Milton; Lovell, Jonathan F.
  • Zhou S; Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY, USA.
  • Yu KOA; Division of Pediatrics Infectious Diseases, Department of Pediatrics, University at Buffalo, Buffalo, NY, USA.
  • Mabrouk MT; Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY, USA.
  • Jahagirdar D; Department of Anatomy and Cell Biology, McGill University, Montreal, Canada.
  • Huang WC; Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY, USA.
  • Guerra JA; Division of Pediatrics Infectious Diseases, Department of Pediatrics, University at Buffalo, Buffalo, NY, USA.
  • He X; Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY, USA.
  • Ortega J; Department of Anatomy and Cell Biology, McGill University, Montreal, Canada.
  • Poole ST; Naval Medical Research Center, Silver Spring, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
  • Hall ER; Naval Medical Research Center, Silver Spring, MD, USA.
  • Gomez-Duarte OG; Division of Pediatrics Infectious Diseases, Department of Pediatrics, University at Buffalo, Buffalo, NY, USA.
  • Maciel M; Naval Medical Research Center, Silver Spring, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA; Department of Microbiology and Immunology, Uniformed Services University Health System, Bethesda, MD, USA. Electronic address: milton.macieljr@gmail.com.
  • Lovell JF; Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY, USA. Electronic address: jflovell@buffalo.edu.
Biomed J ; 46(6): 100588, 2023 Dec.
Article en En | MEDLINE | ID: mdl-36925108
ABSTRACT

BACKGROUND:

Enterotoxigenic Escherichia coli (ETEC) strains cause infectious diarrhea and colonize host intestine epithelia via surface-expressed colonization factors. Colonization factor antigen I (CFA/I), a prevalent ETEC colonization factor, is a vaccine target since antibodies directed to this fimbria can block ETEC adherence and prevent diarrhea.

METHODS:

Two recombinant antigens derived from CFA/I were investigated with a vaccine adjuvant system that displays soluble antigens on the surface of immunogenic liposomes. The first antigen, CfaEB, is a chimeric fusion protein comprising the minor (CfaE) and major (CfaB) subunits of CFA/I. The second, CfaEad, is the adhesin domain of CfaE.

RESULTS:

Owing to their His-tag, recombinant CfaEB and CfaEad, spontaneously bound upon admixture with nanoliposomes containing cobalt-porphyrin phospholipid (CoPoP), as well as a synthetic monophosphoryl lipid A (PHAD) adjuvant. Intramuscular immunization of mice with sub-microgram doses CfaEB or CfaEad admixed with CoPoP/PHAD liposomes elicited serum IgG and intestinal IgA antibodies. The smaller CfaEad antigen benefitted more from liposome display. Serum and intestine antibodies from mice immunized with liposome-displayed CfaEB or CfaEad recognized native CFA/I fimbria as evidenced by immunofluorescence and hemagglutination inhibition assays using the CFA/I-expressing H10407 ETEC strain.

CONCLUSION:

These data show that colonization factor-derived recombinant ETEC antigens exhibit immunogenicity when delivered in immunogenic particle-based formulations.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Infecciones por Escherichia coli / Escherichia coli Enterotoxigénica Límite: Animals Idioma: En Año: 2023 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Infecciones por Escherichia coli / Escherichia coli Enterotoxigénica Límite: Animals Idioma: En Año: 2023 Tipo del documento: Article