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Non-muscle myosin II drives critical steps of nematocyst morphogenesis.
Garg, Niharika; Stibler, Urska Knez; Eismann, Björn; Mercker, Moritz; Bergheim, Bruno Gideon; Linn, Anna; Tuchscherer, Patrizia; Engel, Ulrike; Redl, Stefan; Marciniak-Czochra, Anna; Holstein, Thomas W; Hess, Michael W; Özbek, Suat.
  • Garg N; University of Heidelberg, Centre for Organismal Studies, Department of Molecular Evolution and Genomics, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany.
  • Stibler UK; University of Heidelberg, Centre for Organismal Studies, Department of Molecular Evolution and Genomics, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany.
  • Eismann B; University of Heidelberg, Centre for Organismal Studies, Department of Molecular Evolution and Genomics, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany.
  • Mercker M; Institute for Applied Mathematics, Interdisciplinary Center for Scientific Computing, Heidelberg University, Im Neuenheimer Feld 205, 69120 Heidelberg, Germany.
  • Bergheim BG; University of Heidelberg, Centre for Organismal Studies, Department of Molecular Evolution and Genomics, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany.
  • Linn A; University of Heidelberg, Centre for Organismal Studies, Department of Molecular Evolution and Genomics, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany.
  • Tuchscherer P; University of Heidelberg, Centre for Organismal Studies, Department of Molecular Evolution and Genomics, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany.
  • Engel U; University of Heidelberg, Centre for Organismal Studies, Department of Molecular Evolution and Genomics, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany.
  • Redl S; Nikon Imaging Center at the University of Heidelberg, Bioquant, Heidelberg University, 69120 Heidelberg, Germany.
  • Marciniak-Czochra A; Institute of Neuroanatomy, Medical University of Innsbruck, Müllerstrasse 59, 6020 Innsbruck, Austria.
  • Holstein TW; Institute of Zoology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria.
  • Hess MW; Institute for Applied Mathematics, Interdisciplinary Center for Scientific Computing, Heidelberg University, Im Neuenheimer Feld 205, 69120 Heidelberg, Germany.
  • Özbek S; University of Heidelberg, Centre for Organismal Studies, Department of Molecular Evolution and Genomics, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany.
iScience ; 26(3): 106291, 2023 Mar 17.
Article en En | MEDLINE | ID: mdl-36936784
ABSTRACT
Nematocysts are generated by secretion of proteins into a post-Golgi compartment. They consist of a capsule that elongates into a long tube, which is coiled inside the capsule matrix and expelled during its nano-second discharge deployed for prey capture. The driving force for discharge is an extreme osmotic pressure of 150 bar. The complex processes of tube elongation and invagination under these biomechanical constraints have so far been elusive. Here, we show that a non-muscle myosin II homolog (HyNMII) is essential for nematocyst formation in Hydra. In early nematocysts, HyNMII assembles to a collar around the neck of the protruding tube. HyNMII then facilitates tube outgrowth by compressing it along the longitudinal axis as evidenced by inhibitor treatment and genetic knockdown. In addition, live imaging of a NOWANOWA-GFP transgenic line, which re-defined NOWA as a tube component facilitating invagination, allowed us to analyze the impact of HyNMII on tube maturation.
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2023 Tipo del documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2023 Tipo del documento: Article