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Total chemical synthesis of sumoylated histone H4 reveals negative biochemical crosstalk with histone ubiquitylation.
Singh, Sumeet K; Reyna, Andres; Xie, Xiaowen; Mao, Haibin; Ji, Meihuan; Zheng, Ning; Hsu, Peter L; Chatterjee, Champak.
  • Singh SK; Department of Chemistry, University of Washington, Seattle 98195, USA. champak1@uw.edu.
  • Reyna A; Department of Chemistry, University of Washington, Seattle 98195, USA. champak1@uw.edu.
  • Xie X; Department of Pharmacology, University of Washington; Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA.
  • Mao H; Department of Pharmacology, University of Washington; Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA.
  • Ji M; Department of Chemistry, University of Washington, Seattle 98195, USA. champak1@uw.edu.
  • Zheng N; Department of Pharmacology, University of Washington; Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA.
  • Hsu PL; Department of Pharmacology, University of Washington; Howard Hughes Medical Institute, University of Washington, Seattle 98195, USA.
  • Chatterjee C; Department of Chemistry, University of Washington, Seattle 98195, USA. champak1@uw.edu.
Chem Commun (Camb) ; 59(27): 4063-4066, 2023 Mar 30.
Article en En | MEDLINE | ID: mdl-36938583
ABSTRACT
An efficient total chemical synthesis of site-specifically sumoylated histone H4 was undertaken to generate homogenously modified mononucleosomes. These were tested as substrates in biochemical assays with the histone H2B-specific ubiquitin ligases Rad6 and Bre1, which revealed the strong inhibition of H2B ubiquitylation by SUMO. This novel negative biochemical crosstalk between SUMO and ubiquitin was also confirmed to exist in human cells.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Histonas / Proteínas de Saccharomyces cerevisiae Límite: Humans Idioma: En Año: 2023 Tipo del documento: Article
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Histonas / Proteínas de Saccharomyces cerevisiae Límite: Humans Idioma: En Año: 2023 Tipo del documento: Article