Determination of trypsin using protamine mediated fluorescent enhancement of DNA templated Au nanoclusters.
Mikrochim Acta
; 190(4): 158, 2023 03 27.
Article
en En
| MEDLINE
| ID: mdl-36971858
ABSTRACT
A fluorescent method is described for trypsin determination through the strong electrostatic interactions between cationic polyelectrolytes and single-stranded DNA (ssDNA) templated Au nanoclusters (AuNCs). The ssDNA-AuNCs display improved fluorescence emission with excitation/emission maxima at 280/475 nm after being incorporated with poly(diallyldimethylammonium chloride) (PDDA). Fluorescent enhancement is mainly attributed to the electrostatic interactions occurring between PDDA and ssDNA templates. This can make the conformation of the ssDNA templates to change. Thus, it offers a better microenvironment for stabilizing and protecting ssDNA-AuNCs, and results in fluorescence emission enhancement. By using protamine as a model, the method is employed for the determination of trypsin. The assay enables trypsin to be determined with good sensitivity and a linear response ranging from 5 ngâ
mL-1 to 60 ngâ
mL-1 with a 1.5 ngâ
mL-1 limit of detection. It is also extended to determine the trypsin contents in human's serum samples with recoveries between 98.7% and 103.5% with relative standard deviations (RSDs) between 3.5% and 4.8%. A novel fluorescent strategy has been developed for of trypsin determination by using protamine mediated fluorescent enhancement of DNA templated Au nanoclusters.
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Banco de datos:
MEDLINE
Asunto principal:
Nanopartículas del Metal
Límite:
Humans
Idioma:
En
Año:
2023
Tipo del documento:
Article