Serum starvation affects mitochondrial metabolism of adipose-derived stem/stromal cells.
Cytotherapy
; 25(7): 704-711, 2023 07.
Article
en En
| MEDLINE
| ID: mdl-37061899
ABSTRACT
BACKGROUND AIMS:
A large part of mesenchymal stromal cell (MSC) regenerative and immunomodulatory action is mediated by paracrine signaling. Hence, an increasing body of evidence acknowledges the potential of MSC secretome in a variety of preclinical and clinical scenarios. Mid-term serum deprivation is a common approach in the pipeline of MSC secretome production. Nevertheless, up to now, little is known about the impact of this procedure on the metabolic status of donor cells.METHODS:
Here, through untargeted differential metabolomics, we revealed an impairment of mitochondrial metabolism in adipose-derived MSCs exposed for 72 h to serum deprivation.RESULTS:
This evidence was further confirmed by the significant accumulation of reactive oxygen species and the reduction of succinate dehydrogenase activity. Probably as a repair mechanism, an upregulation of mitochondrial superoxide dismutase was also induced.CONCLUSIONS:
Of note, the analysis of mitochondrial functionality indicated that, despite a significant reduction of basal respiration and ATP production, serum-starved MSCs still responded to changes in energy demand. This metabolic phenotype correlates with the obtained evidence of mitochondrial elongation and branching upon starvation.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Adipocitos
/
Mitocondrias
Límite:
Humans
Idioma:
En
Año:
2023
Tipo del documento:
Article