Constant testing for Pestivirus in cell lines reveals different routes of contamination.
An Acad Bras Cienc
; 95(1): e20220309, 2023.
Article
en En
| MEDLINE
| ID: mdl-37132748
ABSTRACT
Pestivirus can contaminate cell cultures and sera and cause serious problems that evolve the integrity of studies, confidence in diagnostic results, and safety of human and animal vaccines. Contaminations by Pestivirus and other viruses may occur at any time and regular assays of monitoring in cell cultures and your supplies are necessary. This study aimed to analyze the phylogeny of Pestivirus detected from cell cultures, calf serum, and standard strains of three laboratories in Brazil that carry out frequent tests for the monitoring of cellular contaminations. These samples were submitted to phylogenetic analysis to understand the genetic relationship between contaminants occurring in these facilities. As result, the Pestivirus found in samples were Bovine viral diarrhea virus (BVDV-1 and BVDV-2), Hobi-like viruses (often named BVDV-3), and Classical swine fever virus (CSFV), and the phylogenetic analysis help us to infer at three possible routes of contamination in this work.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pestivirus
/
Virus de la Diarrea Viral Bovina
/
Virus de la Diarrea Viral Bovina Tipo 1
Límite:
Animals
/
Humans
Idioma:
En
Año:
2023
Tipo del documento:
Article