Ni(II) functionalized polyhedral oligomeric silsesquioxane based capillary monolith for purification of histidine-tagged proteins by immobilized metal affinity micro-chromatography.

Çambay Kuban, Fatma; Koçer, Ilkay; Kip, Çigdem; Çelik, Eda; Tuncel, Ali

Çambay Kuban, Fatma; Koçer, Ilkay; Kip, Çigdem; Çelik, Eda; Tuncel, Ali.

Çambay Kuban F; Hacettepe University, Graduate School of Science and Engineering, Division of Bioengineering, Ankara, Turkey.
Koçer I; Hacettepe University, Chemical Engineering Department, Ankara, Turkey.
Kip Ç; Hacettepe University, Chemical Engineering Department, Ankara, Turkey.
Çelik E; Hacettepe University, Graduate School of Science and Engineering, Division of Bioengineering, Ankara, Turkey; Hacettepe University, Chemical Engineering Department, Ankara, Turkey.
Tuncel A; Hacettepe University, Graduate School of Science and Engineering, Division of Bioengineering, Ankara, Turkey; Hacettepe University, Chemical Engineering Department, Ankara, Turkey. Electronic address: atuncel@hacettepe.edu.tr.

J Chromatogr B Analyt Technol Biomed Life Sci ; 1225: 123759, 2023 Jun 01.

Article en En | MEDLINE | ID: mdl-37216763

Asunto(s)

Cromatografía de Afinidad; Cromatografía de Afinidad/métodos; Histidina/química; Níquel/química; Compuestos de Organosilicio/química; Proteínas Fluorescentes Verdes/química; Proteínas Fluorescentes Verdes/aislamiento & purificación; Escherichia coli

Palabras clave

Capillary monolith; Green fluorescent protein; His tagged protein; Immobilized metal affinity chromatography; Nano-liquid chromatography

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Cromatografía de Afinidad Idioma: En Año: 2023 Tipo del documento: Article

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Cromatografía de Afinidad Idioma: En Año: 2023 Tipo del documento: Article