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Alzheimer's disease biomarkers in cerebrospinal fluid are stable with the Elecsys immunoassay to most pre-analytical influencing factors except freezing at -80 °C.
Konen, Franz Felix; Maier, Hannah Benedictine; Neyazi, Alexandra; Bleich, Stefan; Neumann, Konstantin; Skripuletz, Thomas.
  • Konen FF; Department of Neurology, Hannover Medical School, Carl-Neuberg-Straße 1, 30625, Hannover, Germany.
  • Maier HB; Department of Psychiatry, Hannover Medical School, Carl-Neuberg-Straße 1, 30625, Hannover, Germany.
  • Neyazi A; Department of Psychiatry, Hannover Medical School, Carl-Neuberg-Straße 1, 30625, Hannover, Germany.
  • Bleich S; Department of Psychiatry, Hannover Medical School, Carl-Neuberg-Straße 1, 30625, Hannover, Germany.
  • Neumann K; Department of Psychiatry and Psychotherapy, Otto-von-Guericke-University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.
  • Skripuletz T; Department of Psychiatry, Hannover Medical School, Carl-Neuberg-Straße 1, 30625, Hannover, Germany.
Neurol Res Pract ; 5(1): 30, 2023 Jun 29.
Article en En | MEDLINE | ID: mdl-37381021
ABSTRACT

BACKGROUND:

Alzheimer´s disease is considered a neurodegenerative disease and is diagnosed by exclusion, while the detection of specific cerebrospinal fluid (CSF) biomarkers, namely amyloid-beta (Aß) peptides Aß1-42 (Aß42), phospho-tau (181P; P-tau), and total-tau (T-tau), has been shown to improve diagnostic accuracy. Recently, a new generation of sample tubes (Sarstedt false-bottom tubes) for the Elecsys CSF immunoassay for the determination of Alzheimer´s disease biomarkers in CSF was introduced, promising better measurability. However, the pre-analytic influencing factors have not yet been sufficiently investigated.

METHODS:

In 29 patients without Alzheimer's disease diagnosis, CSF concentrations of Aß42, P-tau and T-tau were examined in native CSF and after different influencing interventions using the Elecsys immunoassay test method. The following influencing factors were analyzed contamination with blood (10,000 and 20,000 erythrocytes/µl CSF), 14-day storage at 4 °C, blood contamination of CSF and 14-day storage at 4 °C, 14-day freezing at -80 °C in Sarstedt tubes or glass vials, 3-month intermediate storage at -80 °C in glass vials.

RESULTS:

Both storage at -80 °C for 14 days in Sarstedt false-bottom tubes and in glass vials and storage at -80 °C for 3 months in glass vials resulted in significant decreases in Aß42 (13% after 14 days in Sarstedt and 22% in glass vials, 42% after 3 months in glass vials), P-tau (9% after 14 days in Sarstedt and 13% in glass vials, 12% after 3 months in glass vials) and T-tau (12% after 14 days in Sarstedt and 19% in glass vials, 20% after 3 months in glass vials) concentrations in CSF. No significant differences were found for the other pre-analytical influencing factors.

CONCLUSIONS:

Measurements of the concentrations of Aß42, P-tau, and T-tau in CSF with use of the Elecsys immunoassay are robust to the pre-analytical influencing factors of blood contamination and duration of storage. Freezing at -80 °C results in significant reduction of biomarker concentrations regardless of the storage tube and must be considered in retrospective analysis.
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