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Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers.
Liu, Xiao; Lam, Kevin; Zhao, Huayi; Sakane, Sadatsugu; Kim, Hyun Young; Eguileor, Alvaro; Diggle, Karin; Wu, Shuai; Gontijo Weber, Raquel Carvalho; Soroosh, Pejman; Hosseini, Mojgan; Mekeel, Kristin; Brenner, David A; Kisseleva, Tatiana.
  • Liu X; Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA; Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA. Electronic address: xil094@health.ucsd.edu.
  • Lam K; Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
  • Zhao H; Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
  • Sakane S; Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
  • Kim HY; Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
  • Eguileor A; Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
  • Diggle K; Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA; Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
  • Wu S; State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen, China.
  • Gontijo Weber RC; Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA; Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
  • Soroosh P; Janssen Pharmaceutical R&D, Immunometabolism Obesity and Metabolic Disorders, San Diego, CA, USA.
  • Hosseini M; Department of Pathology, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
  • Mekeel K; Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
  • Brenner DA; Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA.
  • Kisseleva T; Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA. Electronic address: tkisseleva@health.ucsd.edu.
STAR Protoc ; 4(3): 102391, 2023 Sep 15.
Article en En | MEDLINE | ID: mdl-37405925
ABSTRACT
Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then detail a liver cell cryopreservation and potential applications, such as the use of human liver cells as a tool to link experimental and translational research.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Enfermedad del Hígado Graso no Alcohólico Límite: Humans Idioma: En Año: 2023 Tipo del documento: Article
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Enfermedad del Hígado Graso no Alcohólico Límite: Humans Idioma: En Año: 2023 Tipo del documento: Article