Treatment of CHO cells with Taxol and reversine improves micronucleation and microcell-mediated chromosome transfer efficiency.

Uno, Narumi; Satofuka, Hiroyuki; Miyamoto, Hitomaru; Honma, Kazuhisa; Suzuki, Teruhiko; Yamazaki, Kyotaro; Ito, Ryota; Moriwaki, Takashi; Hamamichi, Shusei; Tomizuka, Kazuma; Oshimura, Mitsuo; Kazuki, Yasuhiro

Uno, Narumi; Satofuka, Hiroyuki; Miyamoto, Hitomaru; Honma, Kazuhisa; Suzuki, Teruhiko; Yamazaki, Kyotaro; Ito, Ryota; Moriwaki, Takashi; Hamamichi, Shusei; Tomizuka, Kazuma; Oshimura, Mitsuo; Kazuki, Yasuhiro.

Uno N; Laboratory of Bioengineering, Faculty of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Satofuka H; Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.
Miyamoto H; Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.
Honma K; Department of Chromosome Biomedical Engineering, School of Life Science, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.
Suzuki T; Department of Chromosome Biomedical Engineering, School of Life Science, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.
Yamazaki K; Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan.
Ito R; Department of Chromosome Biomedical Engineering, School of Life Science, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.
Moriwaki T; Chromosome Engineering Research Group, The Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan.
Hamamichi S; Laboratory of Bioengineering, Faculty of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Tomizuka K; Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.
Oshimura M; Department of Chromosome Biomedical Engineering, School of Life Science, Faculty of Medicine, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.
Kazuki Y; Chromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.

Mol Ther Nucleic Acids ; 33: 391-403, 2023 Sep 12.

Article en En | MEDLINE | ID: mdl-37547291

Palabras clave

HAC; MAC; MT: Delivery Strategies; Taxol; colcemid; human artificial chromosome; iPSC; microcell-mediated chromosome transfer; mouse artificial chromosome; reversine

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