[Effect of electroacupuncture pretreatment on ferroptosis in neurons of rats with cerebral ischemia-reperfusion injury].
Zhen Ci Yan Jiu
; 48(8): 754-63, 2023 Aug 25.
Article
en Zh
| MEDLINE
| ID: mdl-37614133
ABSTRACT
OBJECTIVE:
To observe the effect of electroacupunctureï¼EAï¼preconditioning on ferroptosis in rats with cerebral ischemia-reperfusion injury ï¼CIRIï¼, so as to explore the neuroprotective mechanism of EA preconditioning.METHODS:
Male SD rats were randomly divided into sham operation, model, EA, inhibitor and inducer groups with 20 rats in each group. The CIRI model was established by modified Zea Longa occlusion of the middle cerebral artery. Before modeling, EA treatment ï¼2 Hz/15 Hz, 1-2 mAï¼ was applied to "Baihui"ï¼GV20ï¼, "Fengfu"ï¼GV16ï¼ and "Dazhui"ï¼GV14ï¼ for rats of the EA group, 20 min a day for 7 consecutive days. Rats of the inhibitor group were intraperitoneally injected with ferristatin-1ï¼25 mg/kgï¼at a slow and uniform rate. Rats of the inducer group were intraperitoneally injected with Erastinï¼100 mg/kgï¼ after 7 days of EA preconditioning, once every 2 h for a total of 4 times. The CIRI models were prepared 2 d later after the above interventions finished by thread-occlusion. The degree of neurological impairment was evaluated by modified Zea Longa score. The percentage of infarct size was calculated by TTC staining. The ultrastructure of neurons in hippocampus was observed by transmission electron microscope. The contents of ferrous ion ï¼Fe2+ï¼, malondialdehyde ï¼MDAï¼ and glutathione ï¼GSHï¼ in cerebral tissue and reactive oxygen species ï¼ROSï¼ in serum were determined by biochemical method. The changes of mitochondrial membrane potential in rats brain tissues were detected by flow cytometry. The mRNA and protein expression levels of glutathione peroxidase 4 ï¼GPX4ï¼, acyl-CoA synthetase long-chain family member 4 ï¼ACSL4ï¼, transferrin receptor ï¼TFRCï¼, 15-lipoxygenase ï¼15-LOXï¼ and cyclooxygenase-2 ï¼COX-2ï¼ in the ischemic hippocampal region of CIRI rats were detected by real-time quantitative PCR and Western blot, respectively.RESULTS:
Compared with the sham operation group, the neurological impairment score, the percentage of cerebral infarction area, the contents of MDA and Fe2+ in cerebral tissue as well as ROS in serum, the protein and mRNA expression levels of ACSL4, TFRC, 15-LOX, COX-2 in hippocampal tissue were increased ï¼P<0.01ï¼, while the content of GSH in cerebral tissue, the protein and mRNA expression levels of GPX4 in hippocampal tissue were decreased ï¼P<0.01ï¼, and mitochondria in brain tissue were significantly damaged ï¼P<0.01ï¼ in the model group. Compared with the model group, the above indexes were all reversed ï¼P<0.05, P<0.01ï¼ in the EA group and inhibitor group. Compared with the EA group, the neurological impairment score, the percentage of cerebral infarction area, the contents of MDA and Fe2+ in cerebral tissue as well as ROS in serum, the protein and mRNA expression le-vels of ACSL4, TFRC, 15-LOX, COX-2 in hippocampal tissue were increased ï¼P<0.05, P<0.01ï¼, while the content of GSH in cerebral tissue, the protein and mRNA expression levels of GPX4 in hippocampal tissue were decreased ï¼P<0.01, P<0.05ï¼, and mitochondria in brain tissue were significantly damaged ï¼P<0.05ï¼ in the inducer group.CONCLUSION:
EA preconditioning has neuroprotective effect on CIRI rats, which may be related to inhibiting ACSL4/TFRC/15-LOX/COX-2 expression and increasing GSH/GPX4 expression.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Daño por Reperfusión
/
Electroacupuntura
/
Ferroptosis
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Año:
2023
Tipo del documento:
Article