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High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9.
Chen, Yudong; Xiang, Haiying; Jia, Ling; Yang, Qianxu; Zhang, Jianduo; Jiang, Jiarui; Zeng, Wanli; Deng, Lele; Jin, Jingjing; Gao, Qian; Li, Xuemei.
  • Chen Y; Yunnan Academy of Tobacco Science, Kunming, 650106, China.
  • Xiang H; Yunnan Academy of Tobacco Science, Kunming, 650106, China.
  • Jia L; Biological Science Research Center, Southwest University, Chongqing, 400715, China.
  • Yang Q; Yunnan Academy of Tobacco Science, Kunming, 650106, China.
  • Zhang J; Yunnan Academy of Tobacco Science, Kunming, 650106, China.
  • Jiang J; Yunnan Academy of Tobacco Science, Kunming, 650106, China.
  • Zeng W; Yunnan Academy of Tobacco Science, Kunming, 650106, China.
  • Deng L; Yunnan Academy of Tobacco Science, Kunming, 650106, China.
  • Jin J; China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China.
  • Gao Q; Yunnan Academy of Tobacco Science, Kunming, 650106, China. gaoqian840905@hotmail.com.
  • Li X; Yunnan Academy of Tobacco Science, Kunming, 650106, China. xmlikm@126.com.
Plant Cell Rep ; 42(12): 2039-2042, 2023 Dec.
Article en En | MEDLINE | ID: mdl-37700173
ABSTRACT
KEY MESSAGE 4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods. Four optimization experiments were established to solve problems encountered during genetic transformation.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Nicotiana / Sistemas CRISPR-Cas Idioma: En Año: 2023 Tipo del documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Nicotiana / Sistemas CRISPR-Cas Idioma: En Año: 2023 Tipo del documento: Article