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Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans.
Scholte, Larissa L S; Leggat, David J; Cohen, Kristen W; Hoeweler, Lara; Erwin, Guacyara C; Rahaman, Farhard; Lombardo, Angela; Philiponis, Vincent; Laufer, Dagna S; Siefers, Heather; Ruppel, Alexis M; Brand, Joshua; Maenza, Janine; Bronson, Rhi; Prabhakaran, Madhu; Jean-Baptiste, Jalen; Kolokythas, Orpheus; Desrosiers, Aimee A; Thoreson, Caroline K; Heit, Antje; Khati, Nadia J; Malkin, Elissa; McElrath, M Juliana; McDermott, Adrian B; Schief, William R; Diemert, David; Bethony, Jeffrey M.
  • Scholte LLS; Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA. Electronic address: larissascholte@gwu.edu.
  • Leggat DJ; The US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
  • Cohen KW; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
  • Hoeweler L; Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
  • Erwin GC; Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
  • Rahaman F; IAVI, New York, NY 10004, USA.
  • Lombardo A; IAVI, New York, NY 10004, USA.
  • Philiponis V; IAVI, New York, NY 10004, USA.
  • Laufer DS; IAVI, New York, NY 10004, USA.
  • Siefers H; IAVI, New York, NY 10004, USA.
  • Ruppel AM; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
  • Brand J; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
  • Maenza J; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA; Department of Medicine, Division of Allergy and Infectious Disease, University of Washington, Seattle, WA 98195, USA.
  • Bronson R; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
  • Prabhakaran M; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
  • Jean-Baptiste J; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
  • Kolokythas O; Department of Radiology, University of Washington, Seattle, WA 98195, USA.
  • Desrosiers AA; Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
  • Thoreson CK; Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
  • Heit A; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
  • Khati NJ; Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
  • Malkin E; Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
  • McElrath MJ; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA; Department of Medicine, Division of Allergy and Infectious Disease, University of Washington, Seattle, WA 98195, USA.
  • McDermott AB; Vaccines Research and Development, Sanofi, Swiftwater, PA 183709, USA.
  • Schief WR; IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA; Center for HIV/AIDS Vaccine Development, The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA; The R
  • Diemert D; Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
  • Bethony JM; Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA.
STAR Protoc ; 4(4): 102576, 2023 Dec 15.
Article en En | MEDLINE | ID: mdl-37733596
ABSTRACT
The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy. For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022).1.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Centro Germinal / Ganglios Linfáticos Límite: Humans Idioma: En Año: 2023 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Centro Germinal / Ganglios Linfáticos Límite: Humans Idioma: En Año: 2023 Tipo del documento: Article