A novel recombinant S-based subunit vaccine induces protective immunity against porcine deltacoronavirus challenge in piglets.
J Virol
; 97(11): e0095823, 2023 Nov 30.
Article
en En
| MEDLINE
| ID: mdl-37846983
ABSTRACT
IMPORTANCE As an emerging porcine enteropathogenic coronavirus that has the potential to infect humans, porcine deltacoronavirus (PDCoV) is receiving increasing attention. However, no effective commercially available vaccines against this virus are available. In this work, we designed a spike (S) protein and receptor-binding domain (RBD) trimer as a candidate PDCoV subunit vaccine. We demonstrated that S protein induced more robust humoral and cellular immune responses than the RBD trimer in mice. Furthermore, the protective efficacy of the S protein was compared with that of inactivated PDCoV vaccines in piglets and sows. Of note, the immunized piglets and suckling pig showed a high level of NAbs and were associated with reduced virus shedding and mild diarrhea, and the high level of NAbs was maintained for at least 4 months. Importantly, we demonstrated that S protein-based subunit vaccines conferred significant protection against PDCoV infection.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de los Porcinos
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Infecciones por Coronavirus
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Coronavirus
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Vacunas de Subunidad
Límite:
Animals
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Female
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Humans
Idioma:
En
Año:
2023
Tipo del documento:
Article