Metabonomics Analysis of Brain Stem Tissue in Rats with Primary Brain Stem Injury Caused Death.
Fa Yi Xue Za Zhi
; 39(4): 373-381, 2023 Aug 25.
Article
en En, Zh
| MEDLINE
| ID: mdl-37859476
ABSTRACT
OBJECTIVES:
To explore the potential biomarkers for the diagnosis of primary brain stem injury (PBSI) by using metabonomics method to observe the changes of metabolites in rats with PBSI caused death.METHODS:
PBSI, non-brain stem brain injury and decapitation rat models were established, and metabolic maps of brain stem were obtained by LC-MS metabonomics method and annotated to the HMDB database. Partial least square-discriminant analysis (PLS-DA) and random forest methods were used to screen potential biomarkers associated with PBSI diagnosis.RESULTS:
Eighty-six potential metabolic markers associated with PBSI were screened by PLS-DA. They were modeled and predicted by random forest algorithm with an accuracy rate of 83.3%. The 818 metabolic markers annotated to HMDB database were used for random forest modeling and prediction, and the accuracy rate was 88.9%. According to the importance in the identification of cause of death, the most important metabolic markers that were significantly up-regulated in PBSI group were HMDB0038126 (genipinic acid, GA), HMDB0013272 (N-lauroylglycine), HMDB0005199 [(R)-salsolinol] and HMDB0013645 (N,N-dimethylsphingosine).CONCLUSIONS:
GA, N-lauroylglycine, (R)-salsolinol and N,N-dimethylsphingosine are expected to be important metabolite indicators in the diagnosis of PBSI caused death, thus providing clues for forensic medicine practice.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Lesiones Encefálicas
/
Metabolómica
Límite:
Animals
Idioma:
En
/
Zh
Año:
2023
Tipo del documento:
Article