Developing a solid-phase method for the enzymatic synthesis of heparan sulfate and chondroitin sulfate backbones.
Glycobiology
; 34(2)2024 Mar 26.
Article
en En
| MEDLINE
| ID: mdl-37995272
ABSTRACT
Despite the recent progress on the solution-phase enzymatic synthesis of heparan sulfate (HS) and chondroitin sulfate (CS), solid-phase enzymatic synthesis has not been fully investigated. Here, we describe the solid-phase enzymatic synthesis of HS and CS backbone oligosaccharides using specialized linkers. We demonstrate the use of immobilized HS linker to synthesize CS, and the use of immobilized CS linker to synthesize HS. The linkers were then digested with chondroitin ABCase and heparin lyases, respectively, to obtain the products. Our findings uncover a potential approach for accelerating the synthesis of structurally homogeneous HS and CS oligosaccharides.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Sulfatos de Condroitina
/
Heparitina Sulfato
Idioma:
En
Año:
2024
Tipo del documento:
Article